| Name | Welan gum |
| Synonyms | welan Biozan Welan gum Fixed gel WELAN GUM Wenlaixiao weilanjiao weilangjiao Kelco Crete |
| CAS | 96949-22-3 |
| Molecular Formula | C7H8N2O3 |
| Molar Mass | 168.152 |
| introduction | dingyou gum is a new type of microbial polysaccharide after xanthan gum, gellan gum and warm wheel gum. it is a water-soluble biopolymer produced by microbial fermentation. it has excellent properties such as thickening, pseudoplastic, high temperature resistance, high salt resistance and wide range of pH adaptability. it can be widely used in petroleum, building materials and other industries, the application prospect is broad. |
| preparation method | a method for extracting dingyou glue from dingyou glue fermentation broth is to extract dingyou glue from the fermentation broth after centrifugation and extraction. Including the following process steps: A, pretreatment of fermentation broth A1, adding lysozyme with enzyme activity of 100,000 u/g to the fermentation broth containing Dingyou gum at pH = 6-8, the concentration of lysozyme in the fermentation broth is 100-200ppm, stirring evenly and maintaining the reaction for 3-5 hours; A2, raise the fermentation broth after reaction in step A1 to 130 ℃-140 ℃ for 5-8 minutes and then reduce it to 60-70 ℃, add acid protease with enzyme activity of 50000u/g, the concentration of acid protease in the fermentation broth is 200-400ppm, and the pretreated fermentation broth is prepared by reacting for 2-4 hours under the conditions of pH = 3-4 and temperature of 50-60 ℃. B, one extraction: mix the two according to the pretreated fermentation broth: ethanol solution with a volume percentage of not less than 85% = 1:0.5-0.6, stir for 30-40 minutes, adjust the pH of the mixed liquid = 8.0-9.5, make Dingyou gum precipitate in flocculent shape; C, primary centrifugation: separate the mixed liquid in step B from solid to liquid to obtain fibrous Dingyou gum; D, secondary extraction: according to the ratio of fibrous Dingyou glue made in step C: ethanol solution with a volume percentage of not less than 85% = 1:0.3-0.5, mix the two and stir for 30-35 minutes, adjust the pH value of the mixed solution to 6.0-6.5, make Dingyou glue precipitate in flocculent shape; E. Secondary centrifugation: separate the mixed liquid made in step D from solid to liquid to obtain fibrous Dingyou glue; F, three elution: according to the fibrous Dingyou glue made in step E: ethanol solution with a volume percentage of not less than 85% = 1:0.4-0.6, mix the two and stir for 30-35 minutes, adjust the pH value of the mixed solution to 6.0-6.5, make Dingyou glue flocculent precipitation, the mixture of solid-liquid separation to obtain fibrous Dingyou glue. |
| use | warm wheel glue is a soluble exopolysaccharide produced by alcaligenes (Alcaligenessp) through aerobic submerged fermentation. due to its good thickening and anti-segregation characteristics, it is used as a good stabilizer and thickening agent in the fields of cement mortar, concrete and other building materials. Used in high molecular polymers, oilfield applications, casting coatings, pigment suspensions, cement or concrete thickeners, tire sealants and coating thickeners. |
| application | warm wheel glue is a solid powder and has good temperature resistance. its aqueous solution has good viscosity and rheological properties, and can withstand high concentration of calcium ions. it can be used as a cement admixture in the cement industry to increase the consistency and rheological properties of ore sand, improve the stability of concrete self-compacting, and increase the pressure and secretion resistance of mud concrete. It can be used in the oil drilling and production industry to provide thickening, suspension and rheological properties for oil mud, and can also be used in fine chemical, daily chemical and other industries. |